最新選精子的科技: 螢光染色PF-1 做試管嬰兒要有好精子和好卵子才有好胚胎，我們都知道好精子，和好卵子意味著精卵染色體都正常，但精子的染色體正不正常怎麼知道？可以考慮用TUNEL染色測驗，如果TUNEL染色綠色表示精子DNA受損，如果TUNEL染色藍色表示正常的染色體，但這樣一來，TUNEL精子活動力通常都少許會受影響?最新的研究是使用PF-1染色法，我們使用一個原理，如果精子DNA受損，代表細胞自我毀滅，代表細胞膜通透性增加，因此 螢光染色染得上去，呈現螢光反應陽性，我們先用PF-1去測試，偵測到精子染色體異常、受損，再用TUNEL來做交叉比對，發現準確率相當的高，而且經過精子染色還是可以用來做試管嬰兒，請看以下照片！ 這恐怕是目前為止科學上上最好選擇精子的方法，我們以前說IMSI、PICSI最近有回顧性、系統性的RCT研究報告，好像沒有什麼用途，最近的這個PF-1染色，提供了未來選擇，精子的新科技。Syst Biol Reprod Med. 2013 Jun;59(3):172-7. doi: 10.3109/19396368.2013.777135. Epub 2013 Apr 8. A novel sorting technology allows for highly efficient selection of sperm without chromatin damage. Funaro MG1, Kim HH, Mazel S, Bolyakov A, Goldstein M, Schlegel PN, Paduch DA. Author information Abstract Sperm chromatin damage has been associated with male infertility, increased risk for spontaneous abortion, and poor embryo development. Available methods for detecting chromatin damage render the sperm no longer suitable for clinical use. Early apoptotic events resulting in chromatin damage are associated with increased permeability of the cell membrane to large ions. We propose the use of a large fluorescent organic cation, proprietary fluorochrome (PF-1), for fluorescence-activated cell sorting (FACS) for negative selection of sperm without chromatin damage. Sperm with chromatin damage are PF-1 positive. Performance of cell sorting by PF-1 was verified with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) after FACS on PF-1(+) and PF-1(-) subpopulations. Whereas 19.5% of PF-1 positive sperm were TUNEL positive only 1.5% sperm in the PF-1(-) fraction were TUNEL positive (p < 0.00001). TUNEL values below 1.9% were considered background fluorescence. Post-sorting motility and vitality were 49.4% (SD: 12.5) and 65.0% (SD: 14.99), respectively. Proprietary fluorochrome activated sperm sorting may decrease or most likely eliminate all of TUNEL positive sperm without adverse effects on viability, providing a new therapeutic avenue for men with a high percentage of TUNEL positive sperm. Further research is needed to determine if the reduction in TUNEL positive sperm using PF-1 will improve in vitro fertilization (IVF) outcomes.